Charmy S Kothari
Nirma University, India
Title: Identification of biomarker(s) from polyherbal formulation used in hyperlipidemia for qualitative and quantitative analysis
Biography
Biography: Charmy S Kothari
Abstract
Herbal medicines have a long therapeutics history and are still serving the need. However, the quality control of herbal medicines still remain a challenge because of the complex nature of the phytoconstituents. The aim and objective of the study is identification of biomarker for qualitative and quantitative analysis of polyherbal formulation containing Arjuna, Guggul & Pushkarmoola in combined dosage form. The poly herbal formulation was evaluated for physical parameters, organoleptic parameters and physicochemical parameters as per AYUSH and WHO guidelines. Quantification of constituent was done by developing and validating High Performance Thin Layer Chromatography (HPTLC). The HPTLC method was developed and validated for poly herbal formulation in the methanol extract. HPTLC separation was achieved on precoated silica gel 60F-254 using Toluene : Ethyl Acetate (8:2 v/v) as mobile phase. Polyherbal formulation methanolic extract gave the peak of E and Z guggulsterones at Rf value 0.34 and 0.41, respectively. Which was further confirmed with reference standard. Assay % of E and Z guggulsterones was found to be 81% and 83%, respectively. Spots of E and Z guggulsterones from polyherbal formulation was isolated for identification. Identification was confirmed by mass spectrometry. Hence, E and Z guggulsterones can be used as a biomarker controlling quality of polyherbal formulation containing Arjuna, Guggul & Pushkarmoola in combined dosage form.